Chapter 5 - Specimen handling and processing. Upon completion of this chapter, you should be able to: List requirements for special specimen handling procedures, describe the technique required for collecting blood cultures, list the steps for Unopette® collection, describe the procedure for performing a microhematocrit,. | Booth, Wallace, and Fitzgerald PowerPoint ® Presentation to Accompany Phlebotomy for Health Care Personnel Chapter 5 Chapter 5 Specimen Processing Specimen Handling and Processing Chapter 5 Objectives Learning Outcomes Upon completion of this chapter, you should be able to: List requirements for special specimen handling procedures. Describe the technique required for collecting blood cultures. List the steps for Unopette® collection. Describe the procedure for performing a microhematocrit. Chapter 5 Objectives cont’d Learning Outcomes (cont'd) Explain the collection process and procedure for making a peripheral blood smear. Discuss common point-of-care and CLIA waived tests. Explain the procedure for urine specimen collection and reagent testing. Identify safety requirements for operating a centrifuge. Chapter 5 Special Handling Procedures Special Specimen Handling Procedures In addition to blood collection, some specimens require special handling either before or . | Booth, Wallace, and Fitzgerald PowerPoint ® Presentation to Accompany Phlebotomy for Health Care Personnel Chapter 5 Chapter 5 Specimen Processing Specimen Handling and Processing Chapter 5 Objectives Learning Outcomes Upon completion of this chapter, you should be able to: List requirements for special specimen handling procedures. Describe the technique required for collecting blood cultures. List the steps for Unopette® collection. Describe the procedure for performing a microhematocrit. Chapter 5 Objectives cont’d Learning Outcomes (cont'd) Explain the collection process and procedure for making a peripheral blood smear. Discuss common point-of-care and CLIA waived tests. Explain the procedure for urine specimen collection and reagent testing. Identify safety requirements for operating a centrifuge. Chapter 5 Special Handling Procedures Special Specimen Handling Procedures In addition to blood collection, some specimens require special handling either before or after the blood is collected. Common Tests Requiring Special Handling Procedures Blood Cultures Cold Agglutinins Chilled and Light-Sensitive Legal Specimens Chapter 5 Blood Cultures Blood Cultures Requested on patients with a high fever Purpose is to isolate microorganisms from the patient’s blood that may be causing the fever Strict aseptic technique is required for the collection of the blood specimen Blood culture bottles are larger than normal venipuncture tubes Chapter 5 Blood Cultures Part 2 Blood culture bottles contain culture media, which enhances the growth of the microorganisms Septicemia is the presence of microorganisms in the blood with symptoms that can result in death Blood cultures are usually ordered STAT Collected in sets of two or three Each set is collected from different sites at different time intervals One sample will be incubated as an anaerobic specimen and the other as an aerobic specimen Blood Cultures Chapter 5 Blood Cultures Part 3 Blood cultures
