From the whole plant of Hedyotis auricularia L., a new glycoside, 1’-deoxy-6’-O-(1- hydroxymethyl-2-hydroxy-1-methoxy)ethylglucopyranoside (1) was isolated along with 1’-O- ethyl- -D-galactopyranoside (2), 2-formyl-5-hydroxymethylfuran (3), stigmasta-5,22-diene-3-O- -D-glucopyranoside (4), ursolic acid (5) and oleanolic acid (6). Among them (1), (2), (3), (4) were the first time known to be present in this plant.