. In this study, 65-bp indel polymorphism in the GOLGB1gene was evaluated in association with body weight in Noi chickens. The live body weight of 170 chickens (90 females, 80 males) at continuous ages of 7-day intervals (from 28 to 84 days) was recorded. A 65-bp indel polymorphism in the GOLGB1 gene was analyzed using the PCR method. | ACADEMIA JOURNAL OF BIOLOGY 2023 45 2 81 88 DOI 2615-9023 18300 ASSOCIATION OF THE 65-BP INDEL POLYMORPHISM IN GOGLB1 GENE WITH BODY WEIGHT OF VIETNAMESE NOI CHICKENS Nguyen Thi Dieu Thuy1 2 Vu Quynh Mai3 Do Vo Anh Khoa4 1 Institute of Biotechnology VAST Vietnam 2 Graduated University of Science and Technology VAST Vietnam 3 VNU University of Science Vietnam 4 Vietnam National University of Forestry at Dong Nai Vietnam Received 26 April 2023 accepted 29 June 2023 ABSTRACT Noi chicken is one of the indigenous fighting-cock breeds of Vietnam with many valuable properties. In chicken the Golgin subfamily B member 1 GOLGB1 gene which locates on chromosome 1 encodes the coat protein 1 vesicle inhibiting factor. A previous study showed that the 65-bp insertion deletion in the GOLGB1 gene was significantly associated with chicken body weight neck weight abdominal fat weight abdominal fat percentage and the yellow index b of the breast in Chinese indigenous chicken N409-breed . In this study 65-bp indel polymorphism in the GOLGB1gene was evaluated in association with body weight in Noi chickens. The live body weight of 170 chickens 90 females 80 males at continuous ages of 7-day intervals from 28 to 84 days was recorded. A 65-bp indel polymorphism in the GOLGB1 gene was analyzed using the PCR method. The results of PCR and sequencing revealed two alleles I and D corresponding to three genotypes II ID DD in which the I allele appears with the highest frequency in the studied population. An association study using Minitab software showed that genotype was significantly associated with body weight in Noi chickens at days 35 to 84 P lt . Individuals with more allele I have higher live body weight than others. Both factors sex and genotype simultaneously affected the body weight of Noi chickens. The results of this study suggested that the 65-bp indel in the GOLGB1 gene could be considered a potential marker for Noi chicken breeding. Keywords Association .
