Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs | J. Vet. Sci. 2008 9 4 367-373 JOURNAL OF Veterinary Science In vitro and in vivo gene therapy with CMV vector-mediated presumed dog p -nerve growth factor in pyridoxine-induced neuropathy dogs 1 12 1 11 1 3 Jin-Young Chung Jung-Hoon Choi Il-Seob Shin Eun-Wha Choi Cheol-Yong Hwang Sang-Koo Lee Hwa-Young Youn1 Departments of Veterinary Internal Medicine and 2Anatomy and Cell Biology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 3Center for Laboratory Animal Science College of Medicine Hanyang University Seoul 133-791 Korea Due to the therapeutic potential of gene therapy for neuronal injury many studies of neurotrophic factors vectors and animal models have been performed. The presumed dog p-nerve growth factor pdp -NGF was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdp -NGF protein reacted with a human p -NGF antibody and showed bioactivity in PC12 cells. The pdp -NGF was shown to have similar bioactivity to the dog p -NGF. The recombinant pdp -NGF plasmid was administrated into the intrathecal space in the gene therapy group. Twenty-four hours after the vector inoculation the gene therapy group and the positive control group were intoxicated with excess pyridoxine for seven days. Each morning throughout the test period the dogs body weight was taken and postural reaction assessments were made. Electrophysiological recordings were performed twice once before the experiment and once after the test period. After the experimental period histological analysis was performed. Dogs in the gene therapy group had no weight change and were normal in postural reaction assessments. Electrophysiological recordings were also normal for the gene therapy group. Histological analysis showed that neither the axons nor the myelin of the dorsal funiculus of L4 were severely damaged in the gene therapy group. In addition the dorsal root ganglia of L4 and the peripheral nerves sciatic nerve did not experience severe
