Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells | Zhang et al. Journal of Experimental Clinical Cancer Research 2011 30 83 http content 30 1 83 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells Ruitao Zhang Huirong Shi Zhimin Chen Qinghua Wu Fang Ren and Haoliang Huang Abstract Background Metastasis-associated in colon cancer 1 MACC1 is demonstrated to be up-regulated in several types of cancer and can serve as biomarker for cancer invasion and metastasis. To investigate the relations between MACC1 and biological processes of ovarian cancer MACC1 specific small hairpin RNA shRNA expression plasmids were used to investigate the effects of MACC1 inhibition on ovarian carcinoma OVCAR-3 cells. Methods Expressions of MACC1 were detected in different ovarian tissues by immunohistochemistry. MACC1 specific shRNA expression plasmids were constructed and transfected into OVCAR-3 cells. Then expressions of MACC1 were examined by reverse transcription polymerase chain reaction RT-PCR and Western blot. Cell proliferation was observed by MTT and monoplast colony formation assay. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Cell migration was assessed by wound healing and transwell migration assay. Matrigel invasion and xenograft model assay were performed to analyze the potential of cell invasion. Activities of Met MEK1 2 ERK1 2 Akt cyclinD1 caspase3 and MMP2 protein were measured by Western blot. Results Overexpressions of MACC1 were detected in ovarian cancer tissues. Expression of MACC1 in OVCAR-3 cells was significantly down-regulated by MACC1 specific small hairpin RNA. In OVCAR-3 cells down-regulation of MACC1 resulted in significant inhibition of cell proliferation migration and invasion meanwhile obvious enhancement of apoptosis. As a consequence of MACC1 knockdown expressions of Met p-MEK1 2 p-ERK1 2 cyclinD1 and MMP2 protein decreased level of .
