Nghiên cứu này trình bày kết quả phân tích tương quan giữa đặc điểm hình thái với chỉ thị trnH-psbA và ITS trong định danh, phân loại loài lan hài Helen (Paphiopedilum helenae Aver.) có nguồn gốc tại Thái Nguyên, Việt Nam. | TNU Journal of Science and Technology 227 05 178 - 185 A STUDY ON THE CORRETATION BETWEEN DNA MARKERS AND MORPHOLOGY CHARACTERISTICS FOR IDENTIFICATION OF Paphiopedilum helenae Aver. OF VIET NAM Nguyen Thi Hai Yen1 Nguyen Dinh Trong2 Ngo Xuan Quang3 4 Do Tien Phat2 4 Chu Hoang Mau5 1TNU - University of Sciences 2VAST - Institute of Biotechnology 3VAST - Institute of Tropical Biology 4VAST - Graduate University of Sciences and Technology 5TNU - University of Education ARTICLE INFO ABSTRACT Received 02 3 2022 Paphiopedilum orchids are loved for their beautiful flowers but are now increasingly depleted in the wild due to over-exploitation. To support bio Revised 21 4 2022 conservation not only the establishment of regulations prohibiting exploitation Published 21 4 2022 and trade it is necessary to prioritize the development of identification and phylogenetic analysis because species of the Paphiopedilum genus have similar KEYWORDS morphology difficult to distinguish when the plant is small or not yet flowering. This study presents the results of correlation analysis between morphological Comparative morphology features and markers trnH-psbA and ITS in the identification and taxonomy of DNA barcode Paphiopedilum helenae Aver. originated in Thai Nguyen Vietnam. The results showed that the identification of Helen s orchid by two markers trnH-psbA and ITS ITS was completely consistent with the comparative morphological method. the Paphiopedilum helenae Aver. DNA barcoding of Paphiopedilum helenae has very high similarity with those in trnH-psbA two species Paphiopedilum tranlienianum and Paphiopedilum barbigerum up to . The molecular phylogeny tree was established by the MEGAX and evolutionary analysis was taken through the Maximum Likelihood method also shows the close relationship of three studied combination of trnH- psbA and ITS markers is a candidate for DNA barcoding for rapid identification of Paphiopedilum helenae Aver. NGHIÊN CỨU SỰ TƯƠNG QUAN
