Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates | J. Vet. Set. 2006 7 3 277-280 JOURNAL OF Veterinary Science The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates Tae Jung Kim1 Jae II Lee2 1 School of Veterinary and Biomedical Sciences Murdoch University Murdoch WA 6150 Australia 2College of Veterinary Medicine Chonnam National University Gwangju 500-757 Korea Using three reference strains ữĩBrachyspira hyodysenteriae B204 B234 B169 one B. pilosicoli 0 43 6 78 one B. murdochii 56-150 one B. intermedia PWS A one B. innocens B256 and ten Korean isolates PCR-RFLP analysis of DNA encoding 23S 1RNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently B. hyodysenteriae and B. pilosicoli revealed different restriction patterns however the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S 1RNA gene sequences between two B. murdochii strains 56-150 and 155-20 were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspừa spp. B. hyodysenteriae and B. pilosicoli as well as an epidemiological tool for characterizing spirochetes isolated from swine. Key words Brachyspira spp. PCR-RFLP 23 s rRNA spứo-chete Introduction Swine dysentery SD is a mucohaemorrhagic colitis of pigs caused by the anaerobic intestinal spirochete Brachyspira hyodysenteriae. Outbreaks of SD are relatively common in a number of developed and developing countries especially where the use of antimicrobial agents is restricted 9 . Until recently there were five species of Brachyspira identified from swine 20 . B. innocens though it is similar to B. hyodysenteriae is non-pathogenic and has been isolated from both healthy and sick pigs this causes confusion in the diagnosis of SD 9 . B. pilosicoli a weakly hemolytic spirochete has been isolated from pigs with mucosal diarrhea 20 . B. intermedia is indole positive with .
