Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia. | Available online http respiratory-research content 3 1 18 Research article Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia MarkT Albrecht1 Wei Wang2 Olga Shamova2 Robert I Lehrer2 3 and Neal L Schiller1 1 Division of Biomedical Sciences University of California Riverside California 92521 USA. 2Department of Medicine University of California Los Angeles Los Angeles California 90095 USA. 3Molecular Biology Institute University of California Los Angeles Los Angeles California 90095 USA. Correspondence Neal L Schiller - Received 1 October 2001 Revisions requested 19 November 2001 Revisions received 29 January 2002 Accepted 31 January 2002 Published 14 March 2002 Respir Res 2002 3 18 2002 Albrecht et al licencee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background Pseudomonas aeruginosa and Burkholderia cepacia infections of cystic fibrosis patients lungs are often resistant to conventional antibiotic therapy. Protegrins are antimicrobial peptides with potent activity against many bacteria including P. aeruginosa. The present study evaluates the correlation between protegrin-1 PG-1 sensitivity resistance and protegrin binding in P. aeruginosa and B. cepacia. Methods The PG-1 sensitivity resistance and PG-1 binding properties of P. aeruginosa and B. cepacia were assessed using radial diffusion assays radioiodinated PG-1 and surface plasmon resonance BiaCore . Results The six P. aeruginosa strains examined were very sensitive to PG-1 exhibiting minimal active concentrations from ug ml in radial diffusion assays. In contrast all five B. cepacia strains examined were greater than 10-fold to 100-fold more resistant with minimal active concentrations ranging from 6 10 ựg ml. When incubated with a radioiodinated variant of PG-1 a sensitive P. aeruginosa strain bound considerably more protegrin molecules per cell than a resistant B. cepacia strain. Binding diffusion and surface plasmon .
