Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Misfolding of CasBrE SU is reversed by interactions with 4070A Env: implications for gammaretroviral neuropathogenesis. | Li and Lynch Retrovirology 2010 7 93 http content 7 1 93 RETR0VIR0L0GY RESEARCH Open Access Misfolding of CasBrE SU is reversed by interactions with 4070A Env implications for gammaretroviral neuropathogenesis Ying Li1 2 3 William P Lynch1 2 Abstract Background CasBrE is a neurovirulent murine leukemia virus MLV capable of inducing paralytic disease with associated spongiform neurodegeneration. The neurovirulence of this virus has been genetically mapped to the surface expressed subunit SU of the env gene. However CasBrE SU synthesized in the absence of the transmembrane subunit TM does not retain ecotropic receptor binding activity indicating that folding of the receptor binding domain RBD requires this domain. Using a neural stem cell NSC based viral trans complementation approach to examine whether misfolded CasBrE SU retained neurovirulence we observed CasBrE SU interaction with the non-neurovirulent amphotropic helper virus 4070A which restored functional activity of CasBrE SU. Results Herein we show that infection of NSCs expressing CasBrE SU with 4070A CasES 4070A-NSCs resulted in the redistribution of CasBrE SU from a strictly secreted product to include retention on the plasma membrane. Cell surface cross-linking analysis suggested that CasBrE SU membrane localization was due to interactions with 4070A Env. Viral particles produced from CasES 4070A-NSCS contained both CasBrE and 4070A gp70 Env proteins. These particles displayed ecotropic receptor-mediated infection but were still 100-fold less efficient than CasE 4070A-NSC virus. Infectious center analysis showed CasBrE SU ecotropic transduction efficiencies approaching those of NSCs expressing full length CasBrE Env CasE SU TM . In addition CasBrE SU-4070A Env interactions resulted in robust ecotropic superinfection interference indicating near native intracellular SU interaction with its receptor mCAT-1. Conclusions In this report we provided evidence that 4070A Env and CasBrE SU .
