Retrovirology Research BioMed Central Open Access Distinct efficacy of HIV-1 entry inhibitors to

Retrovirology Research BioMed Central Open Access Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro Ahidjo Ayouba*, Claude Cannou, Marie-Thérèse Nugeyre, Françoise BarréSinoussi and Elisabeth Menu Address: Institut Pasteur, Département de Virologie, Unité Régulation des Infections Rétrovirales, 25 rue du Dr Roux, 75724 Paris cedex 15, France Email: Ahidjo Ayouba* - ; Claude Cannou - bcannou@; Marie-Thérèse Nugeyre - mnugeyre@; Françoise Barré-Sinoussi - fbarre@; Elisabeth Menu - emenu@ * Corresponding author Published: 31 March 2008 Retrovirology 2008, 5:31 doi: Received: 15 October 2007 Accepted: 31 March 2008 This article is available. | Retrovirology BioMed Central Research Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro Ahidjo Ayouba Claude Cannou Marie-Thérèse Nugeyre Franẹoise Barré-Sinoussi and Elisabeth Menu Open Access Address Institut Pasteur Département de Virologie Unité Régulation des Infections Rétrovirales 25 rue du Dr Roux 75724 Paris cedex 15 France Email Ahidjo Ayouba - Claude Cannou - bcannou@ Marie-Thérèse Nugeyre - mnugeyre@ Franẹoise Barré-Sinoussi - fbarre@ Elisabeth Menu - emenu@ Corresponding author Published 31 March 2008 Received 15 October 2007 Accepted 31 March 2008 Retrovirology 2008 5 31 doi l 742-4690-5-3 I This article is available from http content 5 1 3 1 2008 Ayouba et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background and methods HIV-1 cell-to-cell transmission is more efficient than infection of permissive cells with cell-free particles. The potency of HIV-1 entry inhibitors to inhibit such transmission is not well known. Herein we evaluated the efficacy of this new class of antiretrovirals to block cell-to-cell transmission of HIV-1 in a model of reconstitution of the human placental trophoblast barrier in vitro. Results Our data show that CCR5 antagonists and T20 inhibit the passage of the virus across the BeWo cell monolayer in contact with PBMCs infected with an R5 Ba-L and a dualtropic A204 HIV-1 with IC50s in the range of 100 - 5 000 nM for TAK779 90 to 15 000 nM for .