Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo. | Liu et al. Chinese Medicine 2010 5 25 http content 5 1 25 CHINESE MEDICINE RESEARCH Open Access Tian Xian Liquid TXL induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo -S l-lx T-l 1 2 1 1 Qing Liu Yao Tong Stephen Cho Wing Sze Wing Keung Liu Lam Lam Ellie Shihng Meir Chu Christine Miu Ngan Yow3 Abstract Background Tian Xian Liquid TXL is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo. Method HT-29 colon cancer cells were treated with gradient dilution of TXL. The mitochondrial membrane potential was measured by JC-1 assay. The release of cytochrome c from mitochondrial and apoptosis-related proteins Bax Bcl-2 cleaved caspase-3 9 were examined by Western blot analysis. HT-29 cells were implanted in nude mice to examine the effects of TXL on tumor growth. Result TXL inhibited HT-29 xenografted model and showed a strong and dose-dependent inhibitory effect on the proliferation of HT-29 cells. Mitochondrial membrane potential was reduced by TXL at the concentration of above. For Western blot analysis an increase in Bax expression and a decrease in Bcl-2 expression were observed in TXL-treated cells. TXL treatment increased the protein level ofcleaved casepase-3 and caspase-9 and the release of cytochrome c in cytoplasm was up-regulated as well. Conclusion TXL significantly inhibits cell proliferation in the HT-29 cells and HT-29 xenografted model via the mitochondrial cell death pathway. Background Colorectal carcinoma increased up to four folds in the past decade and the mortality is rising 1 . Much progress has been achieved in alternative medicine 2 such as Chinese medicine. Most methods of chemotherapy for cancer induce cancer cell apoptosis. Excessive apoptosis causes hypotrophy such as ischemic damage whereas insufficient apoptosis leads to uncontrolled .
