Tạo chủng vi khuẩn Agrobacterium tumefaciens mang vector pKO2-PR1 phục vụ công tác xóa gene PR1 ở nấm verticullium dahhliae

Trong nghiên cứu này, chủng vi khuẩn A. tumefacien mang vector xóa gene pKO2- PR1 đã được tạo ra, đây là một công cụ quan trọng để phục vụ công tác xóa gene và nghiên cứu chức năng của gene PR1 trên nấm V. dahliae. | TNU Journal of Science and Technology 228 09 407 - 414 STUDY ON CREATING Agrobacterium tumefaciens STRAINS HARBORING PKO2-PR1 VECTOR FOR PR1 GENE DELETION IN Verticillium dahliae Nguyen Thi Huong1 Dinh Thi Lan1 Ha Van Hieu2 Duong Thi Thanh2 Bui Tri Thuc2 1TNU - University of Science 2TNU - University of Agriculture and Forestry ARTICLE INFO ABSTRACT Received 17 4 2023 Verticillium dahliae is a noxious fungal pathogen that affects plants. It can cause wilting and premature senescence in many crops seriously Revised 19 6 2023 affecting crop productivity. The PR1 gene has been mentioned in Published 19 6 2023 many previous studies which may be related to the initial stage of the host infection process. To serve research on the role of the PR1 gene KEYWORDS in V. dahliae a strain of A. tumefaciens bacteria containing the pKO2- PR1 vector was created. The vector pKO2-PR1 design model was Agrobacterium tumefaciens successfully created using Snapgen Viewer software. The pKO2-PR1 pKO2-PR1 vector contains two segments of sequence before and after the PR1 PR1 gene kb . These segments were amplified by PCR and cloned into a recombination vector. The resulting recombined vector was Gene deletion vector verified by using the XhoI restriction enzyme. The sequence of the Verticillium dahliae pKO2-PR1 vector was fully consistent with the initial design model. The pKO2-PR1 vector was successfully transferred into A. tumefaciens bacteria. The A. tumefaciens strain carrying the pKO2- PR1 vector is an important tool for gene deletion and functional study of the PR1 gene in V. dahliae. TẠO CHỦNG VI KHUẨN Agrobacterium tumefaciens MANG VECTOR PKO2- PR1 PHỤC VỤ CÔNG TÁC XÓA GENE PR1 Ở NẤM Verticullium dahhliae Nguyễn Thị Hương1 Đinh Thị Lan1 Hà Văn Hiếu2 Dương Thị Thanh2 Bùi Tri Thức2 1Trường Đại học Khoa học - ĐH Thái Nguyên 2Trường Đại học Nông Lâm - ĐH Thái Nguyên THÔNG TIN BÀI BÁO TÓM TẮT Ngày nhận bài 17 4 2023 Verticillium dahliae là một loại nấm gây bệnh nguy hại ở thực vật.