In conclusion, sequence analysis may be useful for the rapid detection of STR resistance in MDR M. tuberculosis strains, which in turn could contribute to better control strategies of TB in Vietnam. Other molecular mechanisms associated with STR resistance in STR-resistant strains without mutations in the rpsL and rrs genes need to be further investigated. | ACADEMIA JOURNAL OF BIOLOGY 2023 45 3 87 97 DOI 2615-9023 18156 CHARACTERIZATION OF MUTATIONS CONFERRING STREPTOMYCIN RESISTANCE IN Mycobacterium tuberculosis IN VIETNAM Nguyen Quang Huy1 Anne-Laure Banuls1 2 Nguyen Thi Van Anh3 1 University of Science and Technology of Hanoi Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Ha Noi Vietnam 2 IRD-CNRS-University of Montpellier Montpellier France 3 National Institute of Hygiene and Epidemiology 1 Yec Xanh Ha Noi Vietnam Received 8 March 2023 accepted 12 September 2023 ABSTRACT Recently World Health Organization WHO has listed streptomycin STR in the list of second- line injectable drugs in the multidrug-resistant tuberculosis MDR-TB regimens and may replace amikacin under the same conditions. Nevertheless molecular characterizations associated with STR resistance in the Mycobacterium tuberculosis population have not been fully investigated in Vietnam. The present study aimed to explore the variation and frequency of mutations in rpsL and rrs genes and their relationship with drug-resistant patterns and M. tuberculosis genotypes in 163 STR-resistant strains from Vietnam. The mutation frequency of the rpsL and rrs genes were 62 and respectively and the mutation combination in both genes covered 81 of STR- resistant strains. The most prevalent mutations included rpsL Lys43Arg Lys88Arg rrs A514C and A517C . Thus sequence analysis of rpsL and rrs exhibited a sensitivity of 81 and specificity of 100 for the prediction of STR resistance in Vietnamese M. tuberculosis strains. The prevalence of STR-resistant mutations in double triple and quadruple resistance strains was significantly different compared with mono STR-resistant ones. Similarly mutation frequency associated with STR resistance in MDR strains was significantly higher than that in non-MDR strains. In addition the lineage 2 genotype was significantly correlated with a high rate of STR resistance-conferring mutation as .
