Trọng tâm của nghiên cứu này là đánh giá khả năng phát hiện Bacillus cereus trong thực phẩm bằng môi trường thạch sinh màu RAPID’ (RBC) được so sánh với môi trường thạch chọn lọc tiêu chuẩn Mannitol Egg Yolk Polymyxin (MYP). | Vietnam Journal of Food Control A Scientific Journal of National Institute for Food Control Journal homepage https Research Article Evaluation of the ability to detect Bacillus cereus in food of chromogenic agar media RAPID according to ISO 7932 2004 Vo Thi Nhu Bich1 Truong Huynh Anh Vu2 1 Nong Lam University Ho Chi Minh City Vietnam 2 Center of Analytical Services and Experimentation of Ho Chi Minh City Vietnam Received 07 Nov 2023 Revised 19 Dec 2023 Accepted 10 Jan 2024 Abstract In this study the ability to detect Bacillus cereus of the new chromogenic agar media RAPID RBC was compared with the standard selective culture media Mannitol Egg Yolk Polymyxin MYP recommended by food authorities for isolation identification and quantification of Bacillus cereus. Two types of media were used to conduct the assessment on five categories of food including cereals and cereal products flour and starch vegetables and vegetable products milk and dairy products and confectionery n 164 . The results of the survey Bacillus cereus contamination on RBC MYP of which of the samples didn t meet the standards of the Ministry of Health on the maximum allowable limit of Bacillus cereus in food. Conducting performance testing of RBC according to ISO 16140-2 2016 with parameters growth selectivity specificity relative trueness and accuracy profile the analysis results all met the criteria according to the standard the development PR of the two media were and respectively a high level of background microflora was present on the MYP plates while if it was not the case on the RBC plates both media allowed the detection of Bacillus cereus with a difference of . Compared with the acceptance criteria as prescribed the categories used in the study were all suitable for assessment in the new media with the accuracy between the two methods being equivalent. Keywords Bacillus cereus chromogenic culture media method validation .
