The PCR product on agarose gel showed a clear band at 950 bp. Sequencing method was then adopted and proofread before molecular phylogenetic analysis was applied with reference sequences obtained from the publication of Sung et al. Once again, this analysis confirms the DL0004 specimen as Cordyceps neovolkiana. | Journal of Science and Technology 55 (1A) (2017) 93-98 DOI: ANALYSIS OF nrLSU GENE TO SUPPORT IDENTIFICATION OF FUNGUS BELONGING TO Cordyceps GENUS AND CLAVICIPITACEAE FAMILY Vu Tien Luyen, Lao Duc Thuan2, Trinh Van Hanh2, Dinh Minh Hiep3, Truong Binh Nguyen4, Le Huyen Ai Thuy2, * 1 University of Science, VNU-HCM, 227 Nguyen Van Cu Street, Ho Chi Minh, Viet Nam 2 Ho Chi Minh City Open University, 96 Vo Van Tan Street, Ward 6, District 3, Ho Chi Minh city, Viet Nam 3 Management Board of Agricultural Hi-Tech Park HCMC, 214, D5 Street, Binh Thanh Ward, Ho Chi Minh City, Viet Nam 4 Da Lat University, 1 Phu Dong Thien Vuong Street, Ward 8, Da Lat, Viet Nam * Email: lhathuy@ Received: 30 October 2016; Accepted for publication: 30 May 2017 ABSTRACT Nucleotide sequences of the nuclear large ribosomal subunit (nrLSU) have been used in fungal systematics for a long time. nrLSU was also used in Cordyceps and related genera within the Clavicipitaceae family. A previously identified sample by morphology and ITS was used in this research to analyze the ability of nrLSU to support the identification of entomopathogenic fungi. Our results show that we successfully amplified nrLSU gene using the primer pair LR0R and LR5. The PCR product on agarose gel showed a clear band at 950 bp. Sequencing method was then adopted and proofread before molecular phylogenetic analysis was applied with reference sequences obtained from the publication of Sung et al. Once again, this analysis confirms the DL0004 specimen as Cordyceps neovolkiana. Keywords: Cordyceps, Cordyceps neovolkiana, nrLSU, molecular phylogenetics. 1. INTRODUCTION According to our previous publication [1], the fungal specimen DL0004 was found on rotting leaves, under neotropical forest at the height of 1650 meters at LangBiang Mountain, Lam Dong Province. The fungus has yellow clavate fruitbody with two clear part. The fertile part is pale yellow with clavate head and is 2-3 mm wide .
