Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of the 3’ and 5’ terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts | Kulshreshtha et al. Virology Journal 2010 7 338 http content 7 1 338 VIROLOGY JOURNAL RESEARCH Open Access Identification of the 3 and 5 terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus an orthomyxovirus and evidence for quasispecies based on the non-coding sequences of transcripts 1 1 2 2 2 1 Vikas Kulshreshtha Molly Kibenge Kira Salonius Nathalie Simard Angela Riveroll Frederick Kibenge Abstract Background Infectious salmon anemia ISA virus ISAV is a pathogen of marine-farmed Atlantic salmon Salmo salar a disease first diagnosed in Norway in 1984. This virus which was first characterized following its isolation in cell culture in 1995 belongs to the family Orthomyxoviridae genus Isavirus. The Isavirus genome consists of eight single-stranded RNA segments of negative sense each with one to three open reading frames flanked by 3 and 5 non-coding regions NCRs . Although the terminal sequences of other members of the family Orthomyxoviridae such as Influenzavirus A have been extensively analyzed those of Isavirus remain largely unknown and the few reported are from different ISAV strains and on different ends of the different RNA segments. This paper describes a comprehensive analysis of the 3 and 5 end sequences of the eight RNA segments of ISAV of both European and North American genotypes and evidence of quasispecies of ISAV based on sequence variation in the untranslated regions UTRs of transcripts. Results Two different ISAV strains and two different RNA preparations were used in this study. ISAV strain ADL-PM 3205 ISAV-07 ADL-ISAV-07 of European genotype was the source of total RNA extracted from ISAV-infected TO cells which contained both viral mRNA and cRNA. ISAV strain NBISA01 of North American genotype was the source of vRNA extracted from purified virus. The NCRs of each segment were identified by sequencing cDNA prepared by three different methods 5 RACE Rapid .
