Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Detection and characterization of two chimpanzee polyomavirus genotypes from different subspecies | Deuzing et al. Virology Journal 2010 7 347 http content 7 1 347 VIROLOGY JOURNAL SHORT REPORT Open Access Detection and characterization of two chimpanzee polyomavirus genotypes from different subspecies 1 14 1 2 1 Ilona Deuzing Zahra Fagrouch Marlous J Groenewoud Henk Niphuis Ivanela Kondova Willy Bogers Ernst J Verschoor1 Abstract The complete nucleotide sequences of three chimpanzee polyomavirus genetic variants were determined. Phylogenetic analysis indicated that the viruses form two different genotypes of ChPyV. Comparison with other primate polyomaviruses revealed a putative agnogene and an unusually long VP1 open reading frame. The transcriptional control regions TCR of the viruses were extremely short 155 nucleotides and highly conserved amongst the genotypes. Analysis of the TCR from different chimpanzee subspecies and from a series of tissues from five individuals confirmed its genetic stability and also indicates that double-infections with different genotypes can occur. Findings The number of primate polyomaviruses PyV including human polyomaviruses has rapidly expanded in recent years. Six human viruses KIPyV WUPyV Merkel cell polyomavirus McPyV Trichodysplasia Spinulosa-asso-ciated polyomavirus TSPyV HPyV6 and HPyV7 have been characterized in patients suffering from respiratory tract infections KI and WU Merkel cell carcinomas MC virus-associated trichodysplasia spinulosa TSP or were detected in the skin of healthy individuals HPyV6 and HPyV7 1-5 . Simultaneously novel simian viruses have been discovered in healthy squirrel monkeys and orangutans 6 7 and in diarrheal stool from a chimpanzee 8 . From the chimpanzee polyomavirus ChPyV only the nucleotide sequence of the VP1 gene has been published GenBank AY691168 . We have investigated the genetic variation of ChPyV and sequenced the genome of three chimpanzee polyomavirus variants. We also analyzed the genetic variation of ChPyV in relation to the host subspecies and .
