Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells | Blume et al. BMC Plant Biology 2010 10 29 http 1471-2229 10 29 BMC Plant Biology RESEARCH ARTICLE Open Access Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells 1Ỷ 1Ỷ 1 1 2 2 Yaroslav Blume Alla Yemets Yarina Sheremet Alexey Nyporko Vadym Sulimenko Tetyana Sulimenko Pavel Dráber2 Abstract Background The function of the cortical microtubules composed of ab-tubulin heterodimers is linked to their organizational state which is subject to spatial and temporal modulation by environmental cues. The role of tubulin posttranslational modifications in these processes is largely unknown. Although antibodies against small tubulin regions represent useful tool for studying molecular configuration of microtubules data on the exposure of tubulin epitopes on plant microtubules are still limited. Results Using homology modeling we have generated an Arabidopsis thaliana microtubule protofilament model that served for the prediction of surface exposure of five b-tubulin epitopes as well as tyrosine residues. Peptide scans newly disclosed the position of epitopes detected by antibodies 18D6 b1-10 b426-435 and TU-14 b436-445 . Experimental verification of the results by immunofluorescence microscopy revealed that the exposure of epitopes depended on the mode of fixation. Moreover homology modeling showed that only tyrosines in the C-terminal region of b-tubulins behind b425 were exposed on the microtubule external side. Immunofluorescence microscopy revealed tyrosine phosphorylation of microtubules in plant cells implying that b-tubulins could be one of the targets for tyrosine kinases. Conclusions We predicted surface exposure of five b-tubulin epitopes as well as tyrosine residues on the surface of A. thaliana microtubule protofilament model and validated the obtained results by immunofluorescence microscopy on cortical microtubules in cells. The results suggest that .
