Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Prevalence of a virus similar to human hepatitis B virus in swine | Li et al. Virology Journal 2010 7 60 http content 7 1 60 VIROLOGY JOURNAL RESEARCH Open Access Prevalence of a virus similar to human hepatitis B virus in swine Wengui Li1 2 Ruiping She 1 Liqiang Liu3 Hua You1 Jun Yin1 Abstract Background The objective of this study is to established evidence of the existence of a novel member of the hepadnavirus family endemic in swine. Temporarily this virus was designated as swine hepatitis B virus SHBV . This SHBV can be detected by using human hepatitis B virus diagnostic kits including ELISA immunohistochemical staining and transmission electron microscopy TEM . Also seroprevalence of pig farms in Beijing China and pathological features of SHBV infection was determined. Results Screened result shows that overall prevalence of HBsAg was closed to that of anti-HBsAg whereas HBeAg and anti-HBe were barely detectable. The distribution of HBsAg and HBcAg was examined by immunohistochemistry of liver samples. Typical hepatitis pathological change such as spotty parenchymal cell degeneration necrosis of hepatocytes and proliferation of fibrous connective tissue were observed during histopathological analysis. Analysis of HBsAg-positive serum with TEM revealed two morphologic forms 20 nm and 40 nm sized particles similar to small spherical and Danes particles of HBV. Observation of the ultrastructure of the liver also found HBV-like particles in the nucleus of hepatocytes. Conclusion Our research result implies that SHBV could be a causative agent of swine. The discovery of SHBV will unveil novel evolutionary aspects of hepatitis and provides new information for further hepadnavirus research. Background Viral hepatitis B remain a serious medical challenge worldwide 1 . A strong epidemiological relationship has been established between persistent hepatitis B virus HBV infection and hepatocellular carcinoma HCC 2 . HBV is one of the smallest enveloped animal viruses with a virion diameter of 42 nm. But .
