Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro | Virology Journal BioMed Central Research Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro Berit L Schiotz1 Espen S B kkevold2 Lene C Poulsen1 Siri Mjaaland3 and Tor Gjoen 1 Open Access Address Department of Pharmaceutical Biosciences School of Pharmacy University of Oslo Norway institute of Pathology Rikshospitalet-Radiumhospitalet Medical Center University of Oslo Oslo Norway and 3Department of Food Safety and Infection Biology Norwegian School of Veterinary Science Oslo Norway Email Berit L Schi0tz - Espen S B kkevold - Lene C Poulsen - lenecp@ Siri Mjaaland - Tor Gj0en - Corresponding author Published I July 2009 Received 29 October 2008 Virology Journal 2009 6 91 doi I743-422X-6-91 Accepted 1 July 2009 This article is available from http content 6 I 9I 2009 Schiotz et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Infectious salmon anemia virus ISAV is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia ISA a disease of great importance in the Atlantic salmon farming industry. In vitro ISAV infection causes cytophatic effect CPE in cell lines from Atlantic salmon leading to rounding and finally detachment of the cells from the substratum. In this study we investigated the mode of cell death during in vitro ISAV infection in different Atlantic salmon cell lines using four ISAV strains causing different mortality in vivo. Results The results show that caspase 3 7 activity increased during the course of infection in ASK and SHK-I cells infected cells showed
