Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài:The role of interleukin-12 in the heavy metal-elicited immunomodulation: relevance of various evaluation methods | Journal of Occupational Medicine and Toxicology BioMed Central Research The role of interleukin-12 in the heavy metal-elicited immunomodulation relevance of various evaluation methods Nasr YA Hemdan1 2 3 Open Access Address Fraunhofer Institute for Cell Therapy and Immunology IZI Leipzig Germany institute of Clinical Immunology and Transfusion Medicine - IKIT Faculty of Medicine University of Leipzig Germany and 3Department of Zoology Faculty of Science University of Alexandria Egypt Email Nasr YA Hemdan - Published 6 November 2008 Received 17 June 2008 _ AAAO J . IA I ZZT - or Accepted 6 November 2008 Journal of Occupational Medicine and Toxicology 2008 3 25 doi l745-6673-3-25 This article is available from http content 3 1 25 2008 Hemdan licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Increasing evidence exists that heavy metals modulate T helper cell Th responses and thereby elicit various pathological manifestation. Interleukin IL -12 a crucial innate cytokine was found to be regulated by such xenobiotic agents. This study aimed at testing whether IL-12 profiles may be indicative of heavy metals-induced immunomodulation. Methods Human immunocompetent cells activated either by monoclonal antibodies or heat-killed Salmonella enterica were cultured in the absence or presence of cadmium Cd acetate or mercuric Hg chloride. In vivo experiments were set up where BALB c mice were exposed to sub-lethal doses of Cd or Hg salts for 3 or 5 weeks. Cytotoxicity was assessed by MTT-reduction assay. Modulation of cytokine profiles was evaluated by enzyme-linked immunosorbent assay ELISA cytometric bead-based array CBA and .
