FTuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: luorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging | Nanoscale Res Lett 2009 4 287-295 DOI S11671-008-9239-9 NANO EXPRESS Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging Yuqing Ge Yu Zhang Shiying He Fang Nie Gaojun Teng Ning Gu Received 22 October 2008 Accepted 30 December 2008 Published online 16 January 2009 to the authors 2009 Abstract Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study novel fluorescent magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells SMMC-7721 with the nanoparticles. The nanoparticles exhibited a high affinity to cells which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles concentration. The minimum detected number of labeled cells was around 104 by using a clinical MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use. Keywords Magnetic nanoparticle Fluorescence Chitosan Magnetic resonance imaging Y. Ge Y. Zhang S. He N. Gu El Department of Biological Science and Medical Engineering Jiangsu Laboratory for Biomaterials and Devices State Key Laboratory of Bioelectronics Nanjing People s Republic of China e-mail guning@ F. Nie G. Teng Department of Radiology Zhongda Hospital Southeast University Nanjing 210096 People s Republic of China Introduction Magnetic iron oxide nanoparticles MIONPs have been extensively utilized for drug delivery magnetic resonance imaging MRI hyperthermia techniques
