Xây dựng phương pháp định lượng đồng thời Acid rosmarinic, Sinensetin trong dược liệu Râu mèo (Herba Orthosiphonis spiralis) bằng hệ thống Sắc ký lỏng hiệu năng cao

Mục đích của nghiên cứu này là xây dựng một phương pháp định lượng đồng thời acid rosmarinic, sinensetin trong dược liệu râu mèo (Herba Orthosiphonis spiralis) bằng sắc ký lỏng hiệu năng cao (HPLC). | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 39 No. 2 2023 43-52 Original Article Development of an HPLC Method for Simultaneous Quantification of Rosmarinic Acid Sinensetin in Herba Orthosiphonis spiralis Pham Quoc Tuan1 Nguyen Thi Minh Diep1 Tran Thi Van Anh1 Ha Thanh Hoa1 Nguyen Thanh Hai2 Nguyen Xuan Truong3 Dao Viet Hung1 Ngo Thi Xuan Thinh1 Nguyen Thi Bich Huong1 Hoang Thi Thao3 1 Phu Tho College of Medicine and Pharmacy 2201 Hung Vuong Gia Cam Viet Tri Phu Tho Vietnam 2 VNU School of Medicine and Pharmacy 144 Xuan Thuy Cau Giay Hanoi Vietnam 3 East Asia University of Technology University Village Vo Cuong Bac Ninh Vietnam Received 10 April 2023 Revised 5 May 2023 Accepted 10 June 2023 Abstract The purpose of this study was to develop an HPLC method for the simultaneous quantification of rosmarinic acid and sinensetin in Herba Orthosiphonis spiralis. The chromatographic separation was performed on an Ascentis C8 column 250 mm 5 µm using a gradient elution mode with acetonitrile and water containing formic acid as mobile phase. The flow rate was maintained at mL min. The injection volume was 10 L. The column temperature was set at 40 C. Analytes were detected by a PDA detector at a wavelength of 329 nm. The method was validated for specificity system suitability linearity accuracy precision detection limit and quantitation limit according to ICH and AOAC. The results of validation showed that the method met all the acceptance criteria and was suitable for simultaneous quantification of rosmarinic acid and sinensetin in Herba Orthosiphonis spiralis. Keywords Herba Orthosiphonis spiralis Simultaneous Quantification Sinensetin Rosmarinic acid HPLC. _ Corresponding author. E-mail address phamquoctuan@ https 2588-1132 43 44 P. Q. Tuan et al. VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 39 No. 2 2023 43-52 Xây dựng phương pháp định lượng đồng thời Acid rosmarinic .