Trong phạm vi bài viết này DDT được lựa chọn để đánh giá ảnh hưởng của hóa chất BVTV đến biến đổi cấu trúc của ba gen p53, rarα1, wnt trong phôi và gan cá Medaka O. latipes bằng kỹ thuật TEM và Real Time - PCR | VNU Journal of Science Education Research Vol. 38 No. 3 2022 26-36 Original Article Evaluation of the Toxicity of Dichlorodiphenyl-trichloroethane DDT to the Expression Levels of Three Genes p53 rarα1 wnt in the Embryos and Liver of Fish Medaka Oryzias latipes Tran Thi Thu Huong1 Nguyen Xuan Tong2 1 Hanoi University of Mining and Geology Duc Thang Bac Tu Liem Hanoi Vietnam 2 Industrial University of Ho Chi Minh 12 Nguyen Van Bao Go Vap Ho Chi Minh Vietnam Received 15 May 2021 Revised 02 September 2021 Accepted 22 February 2022 Abstract Three genes p53 rarα1 wnt and liver of Medaka fish tissue were selected to study the toxicity of DDT to fish embryo 8 days old and adult Medaka fish 3 months old . TEM and real-time PCR methods were used to examine changes in structure of liver tissue and genetic expression. The TEM results recorded a clear difference between the control and the experimental sample. Adult Medaka fish exposed to 1 µg L DDT for 24 hours has a distorted liver cell nucleus with many hollow lipid particles and the sinus-shaped meshes are clustered interrupted lysosome is not intact with many degenerative bubbles. The results of real-time PCR analysis showed that DDT affects the development different and proliferation of cells in the embryonic stage and gene expression in adult stage. All three genes p53 rarα1 and wnt in fish embryos changed strongly and tended to be inhibited when exposuring with 1700 µg L DDT the values were recorded respectively and times compared to the control sample 1 times . For adult fish gene expression was lower than fish embryos with real-time PCR analysis values for the three genes p53 rarα1 and wnt respectively and and and times after exposure to 1500 and 1700 µg L DDT. These results demonstrated that gene expression is dependent on the developmental stage of the cell as well as on the dose of the chemical exposed. The development cell differentiation proliferation in the embryonic and
