Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs. | Kato et al. Radiation Oncology 2011 6 116 http content 6 1 116 RADIATION ONCOLOGY RESEARCH Open Access In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays heavy ions and chemotherapeutic drugs I s I rn I I- I I A IX b - 1 2 3 y I r I r-1 I -J s 1 2 4 iri I I I l - 4 A l ir Cl III m r I 1 2 I s r I IX m -J 1 I_I I r I l z-s I I- I I I 11 1 s -J laKamitsu A Kato AKihisa isuda ivntsuru UesaKa AKira Fujimori ladashi Kamada HirohiKo isujii ano Ryuichi OKayasu1 2 Abstract Background Chordoma a rare cancer is usually treated with surgery and or radiation. However very limited characterizations of chordoma cells are available due to a minimal availability only two lines validated by now and the extremely long doubling time. In order to overcome this situation we successfully derived a cell line with a shorter doubling time from the first validated chordoma line U-CH1 and obtained invaluable cell biological data. Method After isolating a subpopulation of U-CH1 cells with a short doubling time U-CH1-N cell growth cell cycle distribution DNA content chromosome number p53 status and cell survival were examined after exposure to X-rays heavy ions camptothecin mitomycin C cisplatin and bleocin. These data were compared with those of HeLa cervical cancer and U87-MG glioblastoma cells. Results The cell doubling times for HeLa U87-MG and U-CH1-N were approximately 18 h 24 h and 3 days respectively. Heavy ion irradiation resulted in more efficient cell Killing than x-rays in all three cell lines. Relative biological effectiveness RBE at 10 survival for U-CH1-N was about for 70 KeV pm carbon and for 200 KeV pm iron ions. Of the four chemicals bleocin showed the most marKed cytotoxic effect on U-CH1-N. Conclusion Our data provide the first comprehensive cellular characterization using cells of chordoma origin and furnish the biological basis for successful clinical results of chordoma treatment by heavy
