Bài viết Mất đoạn lớn và số bản sao DNA ty thể ở bệnh nhân gout được điều trị bằng allopurinol cung cấp thêm thông tin về mất đoạn lớn mtDNA, sự thay đổi số bản sao mtDNA ở người mắc bệnh gout và tìm hiểu mối liên quan của biến đổi này tới bệnh gout và khả năng đáp ứng thuốc điều trị allopurinol ở bệnh nhân gout tại Việt Nam. | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 38 No. 4 2022 96-104 Original Article The Large-scale Deletions and Copy Numbers of Mitochondrial DNA in Gout Patients Treated with Allopurinol Le Lan Phuong1 Tran Le Huyen Linh1 Le Thi Thanh Nhan1 Bui Phuong Thao1 Nguyen Thi Tu Linh1 Tran Huyen Trang2 3 Nguyen Van Hung2 3 Nguyen Thi Van Anh1 Trinh Hong Thai1 1 VNU University of Science 334 Nguyen Trai Thanh Xuan Hanoi Vietnam 2 Hanoi Medical University 1 Ton That Tung Dong Da Hanoi Vietnam 3 Bach Mai Hospital 78 Giai Phong Phuong Mai Dong Da Hanoi Vietnam Received 23 March 2022 Revised 04 May 2022 Accepted 07 May 2022 Abstract Allopurinol is now the first-line treatment for managing gout and its increasing usage has brought more gout patients at risk of developing allopurinol-induced hypersensitive reactions. Up to now our attempts to identify the biomarkers for hypersensitivity to allopurinol are still finite. Besides the association between drug-induced hypersensitive reactions and some mitochondrial DNA mtDNA alterations has been proved but remains unclear in gout patients. In this study 41 blood samples of gout patients prescribed with allopurinol including 22 hypersensitive and 29 tolerant ones were used to analyze their mtDNA large-scale deletions through PCR and determine the mtDNA deletion level and the copy number by quantitative PCR. The results indicated that the mtDNA large-scale deletions and mtDNA copy number of the hypersensitive group were lower than that of the tolerant one p lt . However no association was found between the mtDNA deletion level and the appearance of hypersensitive reactions in gout patients. In addition the study showed that the mtDNA deletion level and copy number were negatively correlated with the copy numbers of mitochondrial DNA in gout patients R p and allopurinol hypersensitive group R p . Thus a decrease in mtDNA copy number might serve as a potential biomarker for further
