Dược liệu Hy thiêm là phần trên mặt đất của cây Hy thiêm, được sử dụng làm thuốc điều trị đau lưng, gối, xương khớp, chân tay tê buốt. Bài viết trình bày định lượng đồng thời acid chlorogenic, acid caffeic và rutin trong dược liệu Hy thiêm bằng phương pháp HPLC. | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 39 No. 2 2023 24-33 Original Article Simultaneous Quantification of Acid chlorogenic Acid caffeic and Rutin in Herba Siegesbeckiae orientalis by HLPC Method Tran Thi Van Anh1 Pham Quoc Tuan1 Nguyen Van Khanh2 Dao Viet Hung1 Nguyen Thanh Hai2 Tran Van Thao3 Nguyen Thi Hong Nhung2 Ngo Thi Xuan Thinh1 Ha Thanh Hoa1 Nguyen Xuan Truong4 1 Phu Tho College of Medicine and Pharmacy 2201 Hung Vuong Gia Cam Viet Tri Phu Tho Vietnam 2 VNU University of Medicine and Pharmacy 144 Xuan Thuy Cau Giay Hanoi Vietnam 3 Golden Dragon Pharmaceutical Co. Ltd 165 Cau Giay Dich Vong Cau Giay Hanoi Vietnam 4 East Asia University of Technology University Village Vo Cuong Bac Ninh Vietnam Received 16 May 2023 Revised 25 May 2023 Accepted 10 June 2023 Abstract Herba Siegesbeckiae is a medicinal material derived from the aerial part of Siegesbeckia orientalis which is used to treat various types of pain such as back knees bones joints and numbness of limbs. This plant contains several phenolic compounds including chlorogenic acid 1 caffeic acid 2 and rutin 3 . They were chosen as marker compounds to develop a quantification method. In this study a high-performance liquid chromatography HPLC method with a DAD detector was used to simultaneously quantify these three compounds 1-3 . The Agilent C18 column 250 x mm 5 µm was used for the separation of the analytes and the mobile phase consisted of MeCN - phosphoric acid in water with gradient elution. The flow rate was ml min and the detection wavelength was 320 nm for 1 and 2 and 360 nm for 3. The injection volume was 20 µl. The method was validated according to the guidelines of ICH and AOAC on system suitability specificity linearity precision and accuracy. The established quantification method was applied to determine the content of compounds 1 2 and 3 in samples of Herba Siegesbeckiae which were found to be mg g mg g mg g respectively. .
