Bài viết Định lượng các chất đánh dấu pinoresinol 4-O-beta-D-glucopyranoside và vladinol F trong dược liệu quả dứa dại Bắc bộ (Pandanus tonkinensis) bằng sắc ký lỏng hiệu năng cao trình bày việc xây dựng và thẩm định phương pháp phân tích định lượng pinoresinol 4-O-beta-D-glucopyranoside và vladinol F trong quả dứa dại Bắc bộ bằng phương pháp HPLC để tiêu chuẩn hóa dược liệu phục vụ mục tiêu sản xuất các chế phẩm theo hướng bảo vệ gan. | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 39 No. 2 2023 34-42 Original Article Determination of Pinoresinol 4-O-beta-D-glucopyranoside and Vladinol F as Markers in Pandanus tonkinensis Fruits by High-Performance Liquid Chromatography Dinh Thi Huyen Trang1 2 Bui Van Trung3 Ngo Quoc Anh4 Duong Hong Anh5 Pham Hung Viet5 1 College of Education Vinh University 182 Le Duan Vinh City Nghe An Vietnam 2 Graduate University of Science and Technology VAST 18 Hoang Quoc Viet Hanoi Vietnam 3 National Institute of Drug Quality Control 20 Hai Ba Trung Hanoi Vietnam 4 Institute of Chemistry VAST 18 Hoang Quoc Viet Hanoi Vietnam 5 VNU University of Science 334 Nguyen Trai Hanoi Vietnam Received 5 May 2023 Revised 09 May 2023 Accepted 10 May 2023 Abstract According to the traditional know-how the Northern wild pineapple Pandanus tonkinensis has been used efficiently as a major component of oriental remedies for liver protection. Based on the experimentally found chemical composition of Pandanus tonkinensis and their obtained biological activities in terms of the hepatoprotection purpose two structure-elucidated compounds pinoresinol 4-O-beta-D-glucopyranoside and vladinol F were selected as potential markers for this medical plant. This paper is aiming to present the validation of the proposed HPLC method for simultaneous quantification of both pinoresinol 4-O-beta-D-glucopyranoside and vladinol F in the fruits of Pandanus tonkinensis with the following conditions using RP-C18 250 mm mm 5 μm column detection wavelength measured at 228 nm the mobile phases consisting of acetonitrile and acetic acid in gradient mode with a flow rate of 1 mL min and the injection volume was 10 L. Method validation showed high specificity linear calibration ranging from to g mL r and from to g mL r for two markers respectively. Besides a good repeatability and intermediate precisions RSD lt 2 a reliable accuracy recovery efficiency between
